The Fischer lab is recruiting a Staff Scientist for a project that exploits the protein conformational landscape for ligand discovery in pediatric cancer.
We are looking for applicants with a strong training in molecular biology, protein biochemistry, and structural biology and a keen interest or ideally experience in ligand discovery.
You will be part of a young, interdisciplinary team that uses structural biology (multi-temperature and high-throughput crystallography) to characterize disease-relevant protein states, computational methods to analyze and exploit them, and experiments (biophysical, biochemical, cell-based assays) to test the impact of small molecules on biology and disease. These approaches have relevance for allosteric regulation of proteins and protein-protein interactions.
The successful candidate has these attributes:
1) Must be able to conduct independent research in molecular biology and protein biochemistry, including cloning and protein purification, that enable structural studies.
2) Demonstrated experience in protein crystallography.
3) Familiarity with large-scale/ high-throughput approaches, automation, liquid handling, biophysical assays or computational approaches is desirable.
4) Experience with _expression_ systems other than E. coliand familiarity with cell-based assays is a plus.
5) Proficiency in e.g. python or shell scripting will facilitate scaling up data analysis.
6) Must be eager to learn and implement new techniques from those relevant areas.
Besides working on your own projects, you will form a liaison for other lab members and
support projects within the lab while maintaining efficient laboratory operations.
Our group is housed in the Department of Chemical Biology and Therapeutics (https://www.stjude.org/research/departments-divisions/chemical-biology-therapeutics.html), and Structural Biology (https://www.stjude.org/research/departments-divisions/structural-biology.html) at St. Jude Children’s Research Hospital. With ongoing investments into exceptional structural and chemical biology facilities, this is an exciting time to join our lab to do cutting-edge research whilst living in an affordable city.
Stable funding for this post is available.
Minimum Experience:
A minimum of five (5) years of relevant and productive (combined) years postdoctoral research associate or five (5) years of combined academic experience at the postdoctoral level or above.
Please direct your questions and application package including a cover letter, current CV, and 3 letters of reference to: Dr. Marcus Fischer (marcus.fischer@stjude.org).
Relevant papers include:
• Fischer et al. (2015). One crystal, two temperatures: cryocooling penalties alter ligand binding to transient protein sites. 1560-64. PMID 26032594
• Merski et al. (2015). Homologous ligands accommodated by discrete conformations of a buried cavity. PNAS 112, 5039-44. PMID 25847998
• Fischer et al. (2014). Incorporation of protein flexibility & conformational energy penalties in docking screens to improve ligand discovery. Nature Chemistry 6, 575-83. PMID 24950326
More info at: https://www.stjude.org/fischer
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