DL/SCI/R34

DATA COLLECTION AND PROCESSING

January, 1993

 

Introduction

1

Invited Speakers’ Contributions

Protein purification and analysis for crystallographic studies

Davis R. Thatcher, Zeneca Pharmaceuticals

2

Novels trends in protein and nucleic acid crystallisation: biochemical and physico-chemical aspects

Richard Geige, Anna Theobald-Dietrich and Bernard Lorber, Strasbourg

12

Crystallisation of membrane proteins

Gerry McDermott, Glasgow

20

Some practical details of data collection at 100K

S.J. Gamblin and D.W. Rogers, Harvard

28

X-ray collimation and generation

U.W. Arndt, MRC Cambridge

33

Autoindexing of rotation diffraction images and parameter refinement

Andrew G.W. Leslie, MRC Cambridge

44

Appendix: Autoindexing in MADNES

J.W. Pflugrath, Cold Spring Harbour

52

Oscillation data reduction program

Zbyszek Otwinowski

56

Recent extensions of the data processing program XDS

Wolfgang Kabsch, Heidelberg

63

LEAP, Laue Evaluation Analysis Package, for time-resolved protein crystallography

Soichi Wakatsuki, Oxford

71

The choice of X-ray wavelength in macromolecular crystallography

John R. Helliwell, Manchester

80

Alternatives in MAD data collection and analysis

Jeffery T. Bolin and Janet L. Smith, Purdue

89

Norman: applications in data analysis

G. David Smith

99

Data collection using imaging plate scanners

Zbigniew Dauter, EMBL, Hamburg

107

Data reduction

P.R. Evans, MRC Cambridge

114

Problematic data collections: give up or persist?

Elspeth F. Garman, Oxford

123

Simple example of the molecular replacement technique: the structure determination of 3-phosphoglycerate kinase from Bacillus stearothermophilus

Gideon J. Davies

132