DL/SCI/R26

IMPROVING PROTEIN PHASES

February, 1988

 

Preface

(iii)

Problems with isomorphous replacement phases

S.E.V. Phillips, Astbury Department of Biophysics, University of Leeds

1

Solvent flattening vs. additional derivative data in the improvement of M.I.R. phases

A.G.W. Leslie, Blackett Laboratory, Imperial College, London

13

A reciprocal space algorithm for calculating molecular envelope using the algorithm of B.C. Wang

A.G.W. Leslie, Blackett Laboratory, Imperial College, London

25

Practical problems of isomorphous replacement

D.M. Blow, K. Henrick and A. Vrielink, Blackett Laboratory, Imperial College, London

32

Iterative molecular averaging and phase refinement of two HLA-A2 crystal forms

M.A. Saper, P.J. Bjorkman and D.C. Wiley, Harvard University

39

Determination of virus structures by the use of molecular replacement density averaging

M.G. Rossman, Purdue University

49

Histogram matching as a density modification technique for phase refinement and extension of protein molecules

Kam Y.J. Zhang and P. Main, University of York

57

Improving protein phases in real space

A.D. Podjarny, IBMC, Strasbourg

65

Improving electron density maps by density modification

E. Dodson, University of York

73

The use of solvent-flattening procedures in the crystal structure determination of quinoprotein methylamine dehydrogenase

F.D.M. Vellieux, H. Groendijk, F. Huitema, M.B.A. Swarte, J. Drenth and W.G.J. Hol, University of Groningen

88

Maximum entropy estimates of the electron density

J. Navaza, Institut Pasteur, Paris

100

Some assorted maximum entropy calculations

R.K. Bryan, EMBL, Heidelberg

107

A practical guide to the use of partial structural phase combination

D.W. Rice , B.F. Anderson and E.N. Baker, Sheffield University and Massey University, New Zealand

113

Use of phase combination in the structure analysis of serum transferrin

H. Jhoti, Birkbeck College

121

Weighting in phase combination

I.J. Tickle, Birkbeck College

130